Entry text : AF009606
[Entry details]
ID AF009606; SV 1; linear; genomic RNA; STD; VRL; 9646 BP.
XX
AC AF009606;
XX
DT 23-AUG-1997 (Rel. 52, Created)
DT 23-JAN-2011 (Rel. 122, Last updated, Version 0)
XX
DE Hepatitis C virus complete genome, complete 5'UTR, complete CDS (Contains:
DE C, E1, E2, P7, NS2, NS3, NS4A, NS4B, NS5A, NS5B), complete 3'UTR.
XX
KW complete genome; complete 5'UTR; complete CDS; C; E1; E2; P7; NS2; NS3;
KW NS4A; NS4B; NS5A; NS5B; complete 3'UTR.
XX
OS Hepatitis C virus subtype 1a
OC Viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae;
OC Hepacivirus.
XX
RN [1]
RP 1-9646
RX DOI; 10.1126/science.277.5325.570.
RX PUBMED; 9228008.
RA Kolykhalov A.A., Agapov E.V., Blight K.J., Mihalik K., Feinstone S.M., Rice
RA C.M.;
RT "Transmission of hepatitis C by intrahepatic inoculation with transcribed
RT RNA";
RL Science 277(5325):570-574(1997).
XX
RN [2]
RP 1-9646
RA Kolykhalov A.A., Rice C.M.;
RT ;
RL Submitted (19-JUN-1997) to the EMBL/GenBank/DDBJ databases.
RL Molecular Microbiology, Washington University School of Medicine, 660 South
RL Euclid Ave., St. Louis, MO 63110-1093, USA
XX
DR GOA; P0C045.
DR InterPro; IPR002522; HCV_core_N.
DR UniProtKB/Swiss-Prot; P0C045; F_HCVH.
XX
CC This entry is part of the european Hepatitis C Virus database (euHCVdb,
CC http://euhcvdb.ibcp.fr). The euHCVdb is funded as part of european
CC contracts HepCVax (EC # QLK2-CT-2002-01329, http://hepcvax.ibcp.fr) and
CC VIRGIL (EC # CT-2004-503359, http://www.virgil-net.org).
XX
FH Key Location/Qualifiers
FH
FT source 1..9646
FT /db_xref="taxon:31646"
FT /db_xref="EMBL:AF009606"
FT /db_xref="euHCVdb:AF009606"
FT /genotype="1a"
FT /genotype_conf="1a"
FT /genotype_prov="1a"
FT /isolate="H77"
FT /mol_type="genomic RNA"
FT /note="assembled from cDNA library representing a
FT population of Hepatitis C virus genomes; viable virus can
FT be reconstituted from this sequence"
FT /organism="Hepatitis C virus subtype 1a"
FT 5'UTR 1..341
FT /function="5'UTR highly conserved genomic region (contains
FT four structural domains (I to IV), domains II to IV make up
FT the internal ribosome entry site (IRES))"
FT /standard_name="5UTR"
FT RBS 44..354
FT /function="The internal ribosome entry site (IRES) is made
FT up by domains II to IV of the 5'UTR"
FT /standard_name="IRES"
FT stem_loop 5..20
FT /function="5'UTR domain I has a regulatory effect for the
FT replication and translation of the genome"
FT /standard_name="5UTR-dI"
FT stem_loop 44..118
FT /function="5'UTR domain II is a component of the IRES"
FT /standard_name="5UTR-dII"
FT stem_loop 125..323
FT /function="5'UTR domain III is a component of the IRES"
FT /standard_name="5UTR-dIII"
FT stem_loop 331..354
FT /function="5'UTR domain IV is a component of the IRES"
FT /standard_name="5UTR-dIV"
FT misc_feature 869..1292
FT /note="C/E1 genotyping region"
FT misc_feature 8276..8615
FT /note="NS5B genotyping region"
FT gene 342..9377
FT /locus_tag="HCVORF1"
FT CDS 342..9377
FT /codon_start=1
FT /db_xref="UniProtKB/Swiss-Prot:P27958"
FT /locus_tag="HCVORF1"
FT /product="Complete HCV polyprotein (contains C, E1, E2, P7,
FT NS2, NS3, NS4A, NS4B, NS5A, NS5B)."
FT /translation="MSTNPKPQRKTKRNTNRRPQDVKFPGGGQIVGGVYLLPRRGPRLG
FT VRATRKTSERSQPRGRRQPIPKARRPEGRTWAQPGYPWPLYGNEGCGWAGWLLSPRGSR
FT PSWGPTDPRRRSRNLGKVIDTLTCGFADLMGYIPLVGAPLGGAARALAHGVRVLEDGVN
FT YATGNLPGCSFSIFLLALLSCLTVPASAYQVRNSSGLYHVTNDCPNSSIVYEAADAILH
FT TPGCVPCVREGNASRCWVAVTPTVATRDGKLPTTQLRRHIDLLVGSATLCSALYVGDLC
FT GSVFLVGQLFTFSPRRHWTTQDCNCSIYPGHITGHRMAWDMMMNWSPTAALVVAQLLRI
FT PQAIMDMIAGAHWGVLAGIAYFSMVGNWAKVLVVLLLFAGVDAETHVTGGSAGRTTAGL
FT VGLLTPGAKQNIQLINTNGSWHINSTALNCNESLNTGWLAGLFYQHKFNSSGCPERLAS
FT CRRLTDFAQGWGPISYANGSGLDERPYCWHYPPRPCGIVPAKSVCGPVYCFTPSPVVVG
FT TTDRSGAPTYSWGANDTDVFVLNNTRPPLGNWFGCTWMNSTGFTKVCGAPPCVIGGVGN
FT NTLLCPTDCFRKHPEATYSRCGSGPWITPRCMVDYPYRLWHYPCTINYTIFKVRMYVGG
FT VEHRLEAACNWTRGERCDLEDRDRSELSPLLLSTTQWQVLPCSFTTLPALSTGLIHLHQ
FT NIVDVQYLYGVGSSIASWAIKWEYVVLLFLLLADARVCSCLWMMLLISQAEAALENLVI
FT LNAASLAGTHGLVSFLVFFCFAWYLKGRWVPGAVYAFYGMWPLLLLLLALPQRAYALDT
FT EVAASCGGVVLVGLMALTLSPYYKRYISWCMWWLQYFLTRVEAQLHVWVPPLNVRGGRD
FT AVILLMCVVHPTLVFDITKLLLAIFGPLWILQASLLKVPYFVRVQGLLRICALARKIAG
FT GHYVQMAIIKLGALTGTYVYNHLTPLRDWAHNGLRDLAVAVEPVVFSRMETKLITWGAD
FT TAACGDIINGLPVSARRGQEILLGPADGMVSKGWRLLAPITAYAQQTRGLLGCIITSLT
FT GRDKNQVEGEVQIVSTATQTFLATCINGVCWTVYHGAGTRTIASPKGPVIQMYTNVDQD
FT LVGWPAPQGSRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSG
FT GPLLCPAGHAVGLFRAAVCTRGVAKAVDFIPVENLETTMRSPVFTDNSSPPAVPQSFQV
FT AHLHAPTGSGKSTKVPAAYAAQGYKVLVLNPSVAATLGFGAYMSKAHGVDPNIRTGVRT
FT ITTGSPITYSTYGKFLADGGCSGGAYDIIICDECHSTDATSILGIGTVLDQAETAGARL
FT VVLATATPPGSVTVSHPNIEEVALSTTGEIPFYGKAIPLEVIKGGRHLIFCHSKKKCDE
FT LAAKLVALGINAVAYYRGLDVSVIPTSGDVVVVSTDALMTGFTGDFDSVIDCNTCVTQT
FT VDFSLDPTFTIETTTLPQDAVSRTQRRGRTGRGKPGIYRFVAPGERPSGMFDSSVLCEC
FT YDAGCAWYELTPAETTVRLRAYMNTPGLPVCQDHLEFWEGVFTGLTHIDAHFLSQTKQS
FT GENFPYLVAYQATVCARAQAPPPSWDQMWKCLIRLKPTLHGPTPLLYRLGAVQNEVTLT
FT HPITKYIMTCMSADLEVVTSTWVLVGGVLAALAAYCLSTGCVVIVGRIVLSGKPAIIPD
FT REVLYQEFDEMEECSQHLPYIEQGMMLAEQFKQKALGLLQTASRQAEVITPAVQTNWQK
FT LEVFWAKHMWNFISGIQYLAGLSTLPGNPAIASLMAFTAAVTSPLTTGQTLLFNILGGW
FT VAAQLAAPGAATAFVGAGLAGAAIGSVGLGKVLVDILAGYGAGVAGALVAFKIMSGEVP
FT STEDLVNLLPAILSPGALVVGVVCAAILRRHVGPGEGAVQWMNRLIAFASRGNHVSPTH
FT YVPESDAAARVTAILSSLTVTQLLRRLHQWISSECTTPCSGSWLRDIWDWICEVLSDFK
FT TWLKAKLMPQLPGIPFVSCQRGYRGVWRGDGIMHTRCHCGAEITGHVKNGTMRIVGPRT
FT CRNMWSGTFPINAYTTGPCTPLPAPNYKFALWRVSAEEYVEIRRVGDFHYVSGMTTDNL
FT KCPCQIPSPEFFTELDGVRLHRFAPPCKPLLREEVSFRVGLHEYPVGSQLPCEPEPDVA
FT VLTSMLTDPSHITAEAAGRRLARGSPPSMASSSASQLSAPSLKATCTANHDSPDAELIE
FT ANLLWRQEMGGNITRVESENKVVILDSFDPLVAEEDEREVSVPAEILRKSRRFARALPV
FT WARPDYNPPLVETWKKPDYEPPVVHGCPLPPPRSPPVPPPRKKRTVVLTESTLSTALAE
FT LATKSFGSSSTSGITGDNTTTSSEPAPSGCPPDSDVESYSSMPPLEGEPGDPDLSDGSW
FT STVSSGADTEDVVCCSMSYSWTGALVTPCAAEEQKLPINALSNSLLRHHNLVYSTTSRS
FT ACQRQKKVTFDRLQVLDSHYQDVLKEVKAAASKVKANLLSVEEACSLTPPHSAKSKFGY
FT GAKDVRCHARKAVAHINSVWKDLLEDSVTPIDTTIMAKNEVFCVQPEKGGRKPARLIVF
FT PDLGVRVCEKMALYDVVSKLPLAVMGSSYGFQYSPGQRVEFLVQAWKSKKTPMGFSYDT
FT RCFDSTVTESDIRTEEAIYQCCDLDPQARVAIKSLTERLYVGGPLTNSRGENCGYRRCR
FT ASGVLTTSCGNTLTCYIKARAACRAAGLQDCTMLVCGDDLVVICESAGVQEDAASLRAF
FT TEAMTRYSAPPGDPPQPEYDLELITSCSSNVSVAHDGAGKRVYYLTRDPTTPLARAAWE
FT TARHTPVNSWLGNIIMFAPTLWARMILMTHFFSVLIARDQLEQALNCEIYGACYSIEPL
FT DLPPIIQRLHGLSAFSLHSYSPGEINRVAACLRKLGVPPLRAWRHRARSVRARLLSRGG
FT RAAICGKYLFNWAVRTKLKLTPIAAAGRLDLSGWFTAGYSGGDIYHSVSHARPRWFWFC
FT LLLLAAGVGIYLLPNR"
FT mat_peptide 342..914
FT /function="C protein coding sequence (c sequence)"
FT /locus_tag="HCVORF1"
FT /product="RNA binding nucleocapsid protein (p21) (capsid)
FT (core protein) (nucleocapsid) (C protein). Produced by
FT proteolytic processing by the host signal peptidases"
FT /prod_ft=(pos:1..191, chain, "C protein")
FT /prod_ft=(pos:1..1, init_met, "Removed from C protein by
FT the cellular aminopeptidase")
FT /standard_name="C"
FT mat_peptide 915..1490
FT /function="E1 protein coding sequence (e1 sequence)"
FT /locus_tag="HCVORF1"
FT /product="Envelope glycoprotein 1 (gp31) (E1 protein).
FT Produced by proteolytic processing by the host signal
FT peptidases. E1 forms heterodimers with the viral E2
FT envelope glycoprotein"
FT /prod_ft=(pos:192..383, chain, "E1 protein")
FT /prod_ft=(pos:353..381, transmem, "Potential transmembrane
FT region of E1")
FT /standard_name="E1"
FT mat_peptide 1491..2579
FT /function="E2 protein coding sequence (e2 sequence)"
FT /locus_tag="HCVORF1"
FT /product="Envelope glycoprotein 2 (GP70) (E2 protein).
FT Produced by proteolytic processing by the host signal
FT peptidases. E2 forms heterodimers with the viral E1
FT envelope glycoprotein"
FT /prod_ft=(pos:384..746, chain, "E2 protein")
FT /prod_ft=(pos:384..410, site, "Hypervariable region 1
FT (HVR1)")
FT /prod_ft=(pos:473..480, site, "Hypervariable region 2
FT (HVR2)")
FT /prod_ft=(pos:659..670, domain, "PKR/eIF2aplha
FT phosporylation homology domain (PePHD)")
FT /prod_ft=(pos:718..741, transmem, "Potential transmembrane
FT region of E2")
FT /standard_name="E2"
FT mat_peptide 2580..2768
FT /function="P7 protein coding sequence (p7 sequence)"
FT /locus_tag="HCVORF1"
FT /product="P7 protein. Produced by proteolytic processing by
FT the host signal peptidases"
FT /prod_ft=(pos:747..809, chain, "P7 protein")
FT /standard_name="P7"
FT mat_peptide 2769..3419
FT /function="NS2 protein coding sequence (ns2 sequence)"
FT /locus_tag="HCVORF1"
FT /product="NS2 protein. Produced by autoproteolytic
FT processing"
FT /prod_ft=(pos:810..1026, chain, "Cysteine protease (NS2
FT protein) (P23)")
FT /prod_ft=(pos:903..1026, model3d, "NS2-3 autoprotease
FT catalytic domain domain (domain I) homology molecular model
FT using template PDB:2hd0")
FT /standard_name="NS2"
FT mat_peptide 3420..5312
FT /function="NS3 protein coding sequence (ns3 sequence)"
FT /locus_tag="HCVORF1"
FT /product="NS3 protein. Produced by autoproteolytic
FT processing"
FT /prod_ft=(pos:1027..1657, chain, "NS3 protein (P70)
FT [Includes: N-ter serine protease; C-ter NTPase and RNA
FT helicase]")
FT /prod_ft=(pos:1027..1206, domain, "NS3 protease domain")
FT /prod_ft=(pos:1213..1657, domain, "NS3 helicase domain")
FT /prod_ft=(pos:1029..1657, model3d, "NS3 protein homology
FT molecular model using template PDB:1cu1A")
FT /standard_name="NS3"
FT mat_peptide 5313..5474
FT /function="NS4A protein coding sequence (ns4a sequence)"
FT /locus_tag="HCVORF1"
FT /product="NS4A protein. Produced by proteolytic processing
FT by the HCV NS3 protease"
FT /prod_ft=(pos:1658..1711, chain, "NS4A protein (P8)")
FT /prod_ft=(pos:1678..1689, domain, "NS3 protease cofactor")
FT /prod_ft=(pos:1678..1689, model3d, "NS4A homology molecular
FT model using template PDB:1cu1Z")
FT /standard_name="NS4A"
FT mat_peptide 5475..6257
FT /function="NS4B protein coding sequence (ns4b sequence)"
FT /locus_tag="HCVORF1"
FT /product="NS4B protein. Produced by proteolytic processing
FT by the HCV NS3 protease"
FT /prod_ft=(pos:1712..1972, chain, "NS4B protein (P27)")
FT /standard_name="NS4B"
FT mat_peptide 6258..7601
FT /function="NS5A protein coding sequence (ns5a sequence)"
FT /locus_tag="HCVORF1"
FT /product="NS5A protein. Produced by proteolytic processing
FT by the HCV NS3 protease. NS5A seems to have a
FT transcriptional activatory role"
FT /prod_ft=(pos:1973..2420, chain, "Serine phosphoprotein
FT (NS5A protein) (P58)")
FT /prod_ft=(pos:2209..2248, site, "Interferon sensitivity
FT determining region (ISDR)")
FT /prod_ft=(pos:2209..2274, domain, "PKR binding domain")
FT /prod_ft=(pos:2326..2334, site, "Nuclear localisation
FT signal (NLS)")
FT /prod_ft=(pos:2356..2379, site, "Variable region V3")
FT /prod_ft=(pos:2008..2170, model3d, "NS5A zinc-binding
FT domain (domain I) homology molecular model using template
FT PDB:1zh1")
FT /standard_name="NS5A"
FT mat_peptide 7602..9374
FT /function="NS5B protein coding sequence (ns5b sequence)"
FT /locus_tag="HCVORF1"
FT /product="NS5B protein. Produced by proteolytic processing
FT by the HCV NS3 protease. NS5B is a RNA-dependent RNA
FT polymerase that plays an essential role in virus
FT replication"
FT /prod_ft=(pos:2421..3011, chain, "RNA-dependent RNA
FT polymerase (NS5B protein) (P68)")
FT /prod_ft=(pos:2423..2986, model3d, "NS5B RNA-dependent RNA
FT polymerase homology molecular model using template PDB:
FT 1c2pB")
FT /standard_name="NS5B"
FT 3'UTR 9378..9646
FT /function="3'UTR (contains a polypyrimidine stretch and the
FT conserved X region)"
FT /standard_name="3UTR"
FT stem_loop 9549..9646
FT /function="3'UTR conserved X region"
FT /standard_name="3UTR-rX"
XX
SQ Sequence 9646 BP; 1889 A; 2893 C; 2724 G; 2140 T; 0 other;
gccagccccc tgatgggggc gacactccac catgaatcac tcccctgtga ggaactactg 60
tcttcacgca gaaagcgtct agccatggcg ttagtatgag tgtcgtgcag cctccaggac 120
cccccctccc gggagagcca tagtggtctg cggaaccggt gagtacaccg gaattgccag 180
gacgaccggg tcctttcttg gataaacccg ctcaatgcct ggagatttgg gcgtgccccc 240
gcaagactgc tagccgagta gtgttgggtc gcgaaaggcc ttgtggtact gcctgatagg 300
gtgcttgcga gtgccccggg aggtctcgta gaccgtgcac catgagcacg aatcctaaac 360
ctcaaagaaa aaccaaacgt aacaccaacc gtcgcccaca ggacgtcaag ttcccgggtg 420
gcggtcagat cgttggtgga gtttacttgt tgccgcgcag gggccctaga ttgggtgtgc 480
gcgcgacgag gaagacttcc gagcggtcgc aacctcgagg tagacgtcag cctatcccca 540
aggcacgtcg gcccgagggc aggacctggg ctcagcccgg gtacccttgg cccctctatg 600
gcaatgaggg ttgcgggtgg gcgggatggc tcctgtctcc ccgtggctct cggcctagct 660
ggggccccac agacccccgg cgtaggtcgc gcaatttggg taaggtcatc gataccctta 720
cgtgcggctt cgccgacctc atggggtaca taccgctcgt cggcgcccct cttggaggcg 780
ctgccagggc cctggcgcat ggcgtccggg ttctggaaga cggcgtgaac tatgcaacag 840
ggaaccttcc tggttgctct ttctctatct tccttctggc cctgctctct tgcctgactg 900
tgcccgcttc agcctaccaa gtgcgcaatt cctcggggct ttaccatgtc accaatgatt 960
gccctaactc gagtattgtg tacgaggcgg ccgatgccat cctgcacact ccggggtgtg 1020
tcccttgcgt tcgcgagggt aacgcctcga ggtgttgggt ggcggtgacc cccacggtgg 1080
ccaccaggga cggcaaactc cccacaacgc agcttcgacg tcatatcgat ctgcttgtcg 1140
ggagcgccac cctctgctcg gccctctacg tgggggacct gtgcgggtct gtctttcttg 1200
ttggtcaact gtttaccttc tctcccaggc gccactggac gacgcaagac tgcaattgtt 1260
ctatctatcc cggccatata acgggtcatc gcatggcatg ggatatgatg atgaactggt 1320
cccctacggc agcgttggtg gtagctcagc tgctccggat cccacaagcc atcatggaca 1380
tgatcgctgg tgctcactgg ggagtcctgg cgggcatagc gtatttctcc atggtgggga 1440
actgggcgaa ggtcctggta gtgctgctgc tatttgccgg cgtcgacgcg gaaacccacg 1500
tcaccggggg aagtgccggc cgcaccacgg ctgggcttgt tggtctcctt acaccaggcg 1560
ccaagcagaa catccaactg atcaacacca acggcagttg gcacatcaat agcacggcct 1620
tgaactgcaa tgaaagcctt aacaccggct ggttagcagg gctcttctat cagcacaaat 1680
tcaactcttc aggctgtcct gagaggttgg ccagctgccg acgccttacc gattttgccc 1740
agggctgggg tcctatcagt tatgccaacg gaagcggcct cgacgaacgc ccctactgct 1800
ggcactaccc tccaagacct tgtggcattg tgcccgcaaa gagcgtgtgt ggcccggtat 1860
attgcttcac tcccagcccc gtggtggtgg gaacgaccga caggtcgggc gcgcctacct 1920
acagctgggg tgcaaatgat acggatgtct tcgtccttaa caacaccagg ccaccgctgg 1980
gcaattggtt cggttgtacc tggatgaact caactggatt caccaaagtg tgcggagcgc 2040
ccccttgtgt catcggaggg gtgggcaaca acaccttgct ctgccccact gattgtttcc 2100
gcaagcatcc ggaagccaca tactctcggt gcggctccgg tccctggatt acacccaggt 2160
gcatggtcga ctacccgtat aggctttggc actatccttg taccatcaat tacaccatat 2220
tcaaagtcag gatgtacgtg ggaggggtcg agcacaggct ggaagcggcc tgcaactgga 2280
cgcggggcga acgctgtgat ctggaagaca gggacaggtc cgagctcagc ccattgctgc 2340
tgtccaccac acagtggcag gtccttccgt gttctttcac gaccctgcca gccttgtcca 2400
ccggcctcat ccacctccac cagaacattg tggacgtgca gtacttgtac ggggtagggt 2460
caagcatcgc gtcctgggcc attaagtggg agtacgtcgt tctcctgttc ctcctgcttg 2520
cagacgcgcg cgtctgctcc tgcttgtgga tgatgttact catatcccaa gcggaggcgg 2580
ctttggagaa cctcgtaata ctcaatgcag catccctggc cgggacgcac ggtcttgtgt 2640
ccttcctcgt gttcttctgc tttgcgtggt atctgaaggg taggtgggtg cccggagcgg 2700
tctacgcctt ctacgggatg tggcctctcc tcctgctcct gctggcgttg cctcagcggg 2760
catacgcact ggacacggag gtggccgcgt cgtgtggcgg cgttgttctt gtcgggttaa 2820
tggcgctgac tctgtcgcca tattacaagc gctacatcag ctggtgcatg tggtggcttc 2880
agtattttct gaccagagta gaagcgcaac tgcacgtgtg ggttcccccc ctcaacgtcc 2940
ggggggggcg cgatgccgtc atcttactca tgtgtgttgt acacccgact ctggtatttg 3000
acatcaccaa actactcctg gccatcttcg gacccctttg gattcttcaa gccagtttgc 3060
ttaaagtccc ctacttcgtg cgcgttcaag gccttctccg gatctgcgcg ctagcgcgga 3120
agatagccgg aggtcattac gtgcaaatgg ccatcatcaa gttaggggcg cttactggca 3180
cctatgtgta taaccatctc acccctcttc gagactgggc gcacaacggc ctgcgagatc 3240
tggccgtggc tgtggaacca gtcgtcttct cccgaatgga gaccaagctc atcacgtggg 3300
gggcagatac cgccgcgtgc ggtgacatca tcaacggctt gcccgtctct gcccgtaggg 3360
gccaggagat actgcttggg ccagccgacg gaatggtctc caaggggtgg aggttgctgg 3420
cgcccatcac ggcgtacgcc cagcagacga gaggcctcct agggtgtata atcaccagcc 3480
tgactggccg ggacaaaaac caagtggagg gtgaggtcca gatcgtgtca actgctaccc 3540
aaaccttcct ggcaacgtgc atcaatgggg tatgctggac tgtctaccac ggggccggaa 3600
cgaggaccat cgcatcaccc aagggtcctg tcatccagat gtataccaat gtggaccaag 3660
accttgtggg ctggcccgct cctcaaggtt cccgctcatt gacaccctgc acctgcggct 3720
cctcggacct ttacctggtc acgaggcacg ccgatgtcat tcccgtgcgc cggcgaggtg 3780
atagcagggg tagcctgctt tcgccccggc ccatttccta cttgaaaggc tcctcggggg 3840
gtccgctgtt gtgccccgcg ggacacgccg tgggcctatt cagggccgcg gtgtgcaccc 3900
gtggagtggc taaggcggtg gactttatcc ctgtggagaa cctagagaca accatgagat 3960
ccccggtgtt cacggacaac tcctctccac cagcagtgcc ccagagcttc caggtggccc 4020
acctgcatgc tcccaccggc agcggtaaga gcaccaaggt cccggctgcg tacgcagccc 4080
agggctacaa ggtgttggtg ctcaacccct ctgttgctgc aacgctgggc tttggtgctt 4140
acatgtccaa ggcccatggg gttgatccta atatcaggac cggggtgaga acaattacca 4200
ctggcagccc catcacgtac tccacctacg gcaagttcct tgccgacggc gggtgctcag 4260
gaggtgctta tgacataata atttgtgacg agtgccactc cacggatgcc acatccatct 4320
tgggcatcgg cactgtcctt gaccaagcag agactgcggg ggcgagactg gttgtgctcg 4380
ccactgctac ccctccgggc tccgtcactg tgtcccatcc taacatcgag gaggttgctc 4440
tgtccaccac cggagagatc cctttttacg gcaaggctat ccccctcgag gtgatcaagg 4500
ggggaagaca tctcatcttc tgccactcaa agaagaagtg cgacgagctc gccgcgaagc 4560
tggtcgcatt gggcatcaat gccgtggcct actaccgcgg tcttgacgtg tctgtcatcc 4620
cgaccagcgg cgatgttgtc gtcgtgtcga ccgatgctct catgactggc tttaccggcg 4680
acttcgactc tgtgatagac tgcaacacgt gtgtcactca gacagtcgat ttcagccttg 4740
accctacctt taccattgag acaaccacgc tcccccagga tgctgtctcc aggactcaac 4800
gccggggcag gactggcagg gggaagccag gcatctacag atttgtggca ccgggggagc 4860
gcccctccgg catgttcgac tcgtccgtcc tctgtgagtg ctatgacgcg ggctgtgctt 4920
ggtatgagct cacgcccgcc gagactacag ttaggctacg agcgtacatg aacaccccgg 4980
ggcttcccgt gtgccaggac catcttgaat tttgggaggg cgtctttacg ggcctcactc 5040
atatagatgc ccactttcta tcccagacaa agcagagtgg ggagaacttt ccttacctgg 5100
tagcgtacca agccaccgtg tgcgctaggg ctcaagcccc tcccccatcg tgggaccaga 5160
tgtggaagtg tttgatccgc cttaaaccca ccctccatgg gccaacaccc ctgctataca 5220
gactgggcgc tgttcagaat gaagtcaccc tgacgcaccc aatcaccaaa tacatcatga 5280
catgcatgtc ggccgacctg gaggtcgtca cgagcacctg ggtgctcgtt ggcggcgtcc 5340
tggctgctct ggccgcgtat tgcctgtcaa caggctgcgt ggtcatagtg ggcaggattg 5400
tcttgtccgg gaagccggca attatacctg acagggaggt tctctaccag gagttcgatg 5460
agatggaaga gtgctctcag cacttaccgt acatcgagca agggatgatg ctcgctgagc 5520
agttcaagca gaaggccctc ggcctcctgc agaccgcgtc ccgccaagca gaggttatca 5580
cccctgctgt ccagaccaac tggcagaaac tcgaggtctt ctgggcgaag cacatgtgga 5640
atttcatcag tgggatacaa tacttggcgg gcctgtcaac gctgcctggt aaccccgcca 5700
ttgcttcatt gatggctttt acagctgccg tcaccagccc actaaccact ggccaaaccc 5760
tcctcttcaa catattgggg gggtgggtgg ctgcccagct cgccgccccc ggtgccgcta 5820
ccgcctttgt gggcgctggc ttagctggcg ccgccatcgg cagcgttgga ctggggaagg 5880
tcctcgtgga cattcttgca gggtatggcg cgggcgtggc gggagctctt gtagcattca 5940
agatcatgag cggtgaggtc ccctccacgg aggacctggt caatctgctg cccgccatcc 6000
tctcgcctgg agcccttgta gtcggtgtgg tctgcgcagc aatactgcgc cggcacgttg 6060
gcccgggcga gggggcagtg caatggatga accggctaat agccttcgcc tcccggggga 6120
accatgtttc ccccacgcac tacgtgccgg agagcgatgc agccgcccgc gtcactgcca 6180
tactcagcag cctcactgta acccagctcc tgaggcgact gcatcagtgg ataagctcgg 6240
agtgtaccac tccatgctcc ggttcctggc taagggacat ctgggactgg atatgcgagg 6300
tgctgagcga ctttaagacc tggctgaaag ccaagctcat gccacaactg cctgggattc 6360
cctttgtgtc ctgccagcgc gggtataggg gggtctggcg aggagacggc attatgcaca 6420
ctcgctgcca ctgtggagct gagatcactg gacatgtcaa aaacgggacg atgaggatcg 6480
tcggtcctag gacctgcagg aacatgtgga gtgggacgtt ccccattaac gcctacacca 6540
cgggcccctg tactcccctt cctgcgccga actataagtt cgcgctgtgg agggtgtctg 6600
cagaggaata cgtggagata aggcgggtgg gggacttcca ctacgtatcg ggtatgacta 6660
ctgacaatct taaatgcccg tgccagatcc catcgcccga atttttcaca gaattggacg 6720
gggtgcgcct acataggttt gcgccccctt gcaagccctt gctgcgggag gaggtatcat 6780
tcagagtagg actccacgag tacccggtgg ggtcgcaatt accttgcgag cccgaaccgg 6840
acgtagccgt gttgacgtcc atgctcactg atccctccca tataacagca gaggcggccg 6900
ggagaaggtt ggcgagaggg tcaccccctt ctatggccag ctcctcggcc agccagctgt 6960
ccgctccatc tctcaaggca acttgcaccg ccaaccatga ctcccctgac gccgagctca 7020
tagaggctaa cctcctgtgg aggcaggaga tgggcggcaa catcaccagg gttgagtcag 7080
agaacaaagt ggtgattctg gactccttcg atccgcttgt ggcagaggag gatgagcggg 7140
aggtctccgt acccgcagaa attctgcgga agtctcggag attcgcccgg gccctgcccg 7200
tttgggcgcg gccggactac aaccccccgc tagtagagac gtggaaaaag cctgactacg 7260
aaccacctgt ggtccatggc tgcccgctac cacctccacg gtcccctcct gtgcctccgc 7320
ctcggaaaaa gcgtacggtg gtcctcaccg aatcaaccct atctactgcc ttggccgagc 7380
ttgccaccaa aagttttggc agctcctcaa cttccggcat tacgggcgac aatacgacaa 7440
catcctctga gcccgcccct tctggctgcc cccccgactc cgacgttgag tcctattctt 7500
ccatgccccc cctggagggg gagcctgggg atccggatct cagcgacggg tcatggtcga 7560
cggtcagtag tggggccgac acggaagatg tcgtgtgctg ctcaatgtct tattcctgga 7620
caggcgcact cgtcaccccg tgcgctgcgg aagaacaaaa actgcccatc aacgcactga 7680
gcaactcgtt gctacgccat cacaatctgg tgtattccac cacttcacgc agtgcttgcc 7740
aaaggcagaa gaaagtcaca tttgacagac tgcaagttct ggacagccat taccaggacg 7800
tgctcaagga ggtcaaagca gcggcgtcaa aagtgaaggc taacttgcta tccgtagagg 7860
aagcttgcag cctgacgccc ccacattcag ccaaatccaa gtttggctat ggggcaaaag 7920
acgtccgttg ccatgccaga aaggccgtag cccacatcaa ctccgtgtgg aaagaccttc 7980
tggaagacag tgtaacacca atagacacta ccatcatggc caagaacgag gttttctgcg 8040
ttcagcctga gaaggggggt cgtaagccag ctcgtctcat cgtgttcccc gacctgggcg 8100
tgcgcgtgtg cgagaagatg gccctgtacg acgtggttag caagctcccc ctggccgtga 8160
tgggaagctc ctacggattc caatactcac caggacagcg ggttgaattc ctcgtgcaag 8220
cgtggaagtc caagaagacc ccgatggggt tctcgtatga tacccgctgt tttgactcca 8280
cagtcactga gagcgacatc cgtacggagg aggcaattta ccaatgttgt gacctggacc 8340
cccaagcccg cgtggccatc aagtccctca ctgagaggct ttatgttggg ggccctctta 8400
ccaattcaag gggggaaaac tgcggctacc gcaggtgccg cgcgagcggc gtactgacaa 8460
ctagctgtgg taacaccctc acttgctaca tcaaggcccg ggcagcctgt cgagccgcag 8520
ggctccagga ctgcaccatg ctcgtgtgtg gcgacgactt agtcgttatc tgtgaaagtg 8580
cgggggtcca ggaggacgcg gcgagcctga gagccttcac ggaggctatg accaggtact 8640
ccgccccccc cggggacccc ccacaaccag aatacgactt ggagcttata acatcatgct 8700
cctccaacgt gtcagtcgcc cacgacggcg ctggaaagag ggtctactac cttacccgtg 8760
accctacaac ccccctcgcg agagccgcgt gggagacagc aagacacact ccagtcaatt 8820
cctggctagg caacataatc atgtttgccc ccacactgtg ggcgaggatg atactgatga 8880
cccatttctt tagcgtcctc atagccaggg atcagcttga acaggctctt aactgtgaga 8940
tctacggagc ctgctactcc atagaaccac tggatctacc tccaatcatt caaagactcc 9000
atggcctcag cgcattttca ctccacagtt actctccagg tgaaatcaat agggtggccg 9060
catgcctcag aaaacttggg gtcccgccct tgcgagcttg gagacaccgg gcccggagcg 9120
tccgcgctag gcttctgtcc agaggaggca gggctgccat atgtggcaag tacctcttca 9180
actgggcagt aagaacaaag ctcaaactca ctccaatagc ggccgctggc cggctggact 9240
tgtccggttg gttcacggct ggctacagcg ggggagacat ttatcacagc gtgtctcatg 9300
cccggccccg ctggttctgg ttttgcctac tcctgctcgc tgcaggggta ggcatctacc 9360
tcctccccaa ccgatgaagg ttggggtaaa cactccggcc tcttaggcca tttcctgttt 9420
tttttttttt tttttttttt tttttttttt tttttttttt ttttttttct tttttttttt 9480
ttttttcctt tttttttttt ttttttttct ttccttcttt tttcctttct tttccttcct 9540
tctttaatgg tggctccatc ttagccctag tcacggctag ctgtgaaagg tccgtgagcc 9600
gcatgactgc agagagtgct gatactggcc tctctgcaga tcatgt 9646
//
