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The Nobel Prize in Physiology or Medicine 2020 "for the discovery of Hepatitis C virus".

Entry details : D00944

[General] [References] [Features] [Sequences] [Text Entry]    [LANL]
GENERAL INFORMATION
ID D00944; SV 1; linear; genomic RNA; STD; VRL; 9589 BP.
Primary AC D00944
Creation Date 03-FEB-1992 (Rel. 30, Created)
Update Date 23-JAN-2011 (Rel. 122, Last updated , Version 0)
Description Hepatitis C virus partial genome, complete 5'UTR, complete CDS (Contains: C, E1, E2, P7, NS2, NS3, NS4A, NS4B, NS5A, NS5B), partial 3'UTR.
Genotype
Deposited 2a
Provisional 2a
Confirmed 2a
Isolate HC-J6
Keywords partial genome;  complete 5'UTR;  complete CDS;  C;  E1;  E2;  P7;  NS2;  NS3;  NS4A;  NS4B;  NS5A;  NS5B;  partial 3'UTR; 
Organism
OS Hepatitis C virus
OC Viruses;  ssRNA positive-strand viruses, no DNA stage;  Flaviviridae;  Hepacivirus; 
[General] [References] [Features] [Sequences] [Text Entry]    [LANL]
REFERENCES
[1]
1 - 9589
RA Okamoto H.;  Okada S.;  Sugiyama Y.;  Kurai K.;  Iizuka H.;  Machida A.;  Miyakawa Y.;  Mayumi M.; 
RT Nucleotide sequences of the genomic RNA of hepatitis C virus isolated from a human carrier: comparison with reported isolates for conserved and divergent regions
RL J. Gen. Virol. 72:2697-2704(1991).
RX PUBMED: 1658196  
[2]
RA Han J.H.;  Houghton M.; 
RT Group specific sequences and conserved secondary structures at the 3' end of HCV genome and its implication for viral replication
RL Nucleic Acids Res. 20(13):3520-3520(1992).
RX DOI: 10.1093/nar/20.13.3520  
PUBMED: 1321416  
[3]
RA Hotta H.;  Doi H.;  Hayashi T.;  Purwanta M.;  Soemarto W.;  Mizokami M.;  Ohba K.;  Homma M.; 
RT Analysis of the core and E1 envelope region sequences of a novel variant of hepatitis C virus obtained in Indonesia
RL Arch. Virol. 136(1-2):53-62(1994).
RX DOI: 10.1007/BF01538816  
PUBMED: 7545932  
[General] [References] [Features] [Sequences] [Text Entry]    [LANL]
FEATURES
source
 1..9589
(9589)
db_xref taxon : 11103
db_xref EMBL : D00944
db_xref euHCVdb : AF009606
mol_type genomic RNA
organism Hepatitis C virus
5'UTR
 1..340
(340)

5UTR
function 5'UTR highly conserved genomic region (contains four structural domains (I to IV), domains II to IV make up the internal ribosome entry site (IRES))
standard_name 5UTR
RBS
 43..353
(311)

IRES
function The internal ribosome entry site (IRES) is made up by domains II to IV of the 5'UTR
standard_name IRES
stem_loop
 5..19
(15)

5UTR-dI
function 5'UTR domain I has a regulatory effect for the replication and translation of the genome
standard_name 5UTR-dI
stem_loop
 43..117
(75)

5UTR-dII
function 5'UTR domain II is a component of the IRES
standard_name 5UTR-dII
stem_loop
 124..322
(199)

5UTR-dIII
function 5'UTR domain III is a component of the IRES
standard_name 5UTR-dIII
stem_loop
 330..353
(24)

5UTR-dIV
function 5'UTR domain IV is a component of the IRES
standard_name 5UTR-dIV
misc_feature
 868..1291
(424)

5UTR-dIV
note C/E1 genotyping region
misc_feature
 8341..8680
(340)

5UTR-dIV
note NS5B genotyping region
gene
 341..9442
(9102)

5UTR-dIV
locus_tag HCVORF1
CDS
 341..9442
(9102)

5UTR-dIV
codon_start offset : 1
db_xref UniProtKB/Swiss-Prot : P26660
locus_tag HCVORF1
product Complete HCV polyprotein (contains C, E1, E2, P7, NS2, NS3, NS4A, NS4B, NS5A, NS5B).
translation VIEW
mat_peptide
 341..913
(573)

C
function C protein coding sequence (c sequence)
locus_tag HCVORF1
product RNA binding nucleocapsid protein (p21) (capsid) (core protein) (nucleocapsid) (C protein). Produced by proteolytic processing by the host signal peptidases
prod_ft
chain 1..191  (191) C protein
prod_ft
init_met 1..1  (1) Removed from C protein by the cellular aminopeptidase
standard_name C
mat_peptide
 914..1489
(576)

E1
function E1 protein coding sequence (e1 sequence)
locus_tag HCVORF1
product Envelope glycoprotein 1 (gp31) (E1 protein). Produced by proteolytic processing by the host signal peptidases. E1 forms heterodimers with the viral E2 envelope glycoprotein
prod_ft
chain 192..383  (192) E1 protein
prod_ft
transmem 353..381  (29) Potential transmembrane region of E1
standard_name E1
mat_peptide
 1490..2590
(1101)

E2
function E2 protein coding sequence (e2 sequence)
locus_tag HCVORF1
product Envelope glycoprotein 2 (GP70) (E2 protein). Produced by proteolytic processing by the host signal peptidases. E2 forms heterodimers with the viral E1 envelope glycoprotein
prod_ft
chain 384..750  (367) E2 protein
prod_ft
site 384..410  (27) Hypervariable region 1 (HVR1)
prod_ft
site 473..481  (9) Hypervariable region 2 (HVR2)
prod_ft
domain 663..674  (12) PKR/eIF2aplha phosporylation homology domain (PePHD)
prod_ft
transmem 722..745  (24) Potential transmembrane region of E2
standard_name E2
mat_peptide
 2591..2779
(189)

P7
function P7 protein coding sequence (p7 sequence)
locus_tag HCVORF1
product P7 protein. Produced by proteolytic processing by the host signal peptidases
prod_ft
chain 751..813  (63) P7 protein
standard_name P7
mat_peptide
 2780..3430
(651)

NS2
function NS2 protein coding sequence (ns2 sequence)
locus_tag HCVORF1
product NS2 protein. Produced by autoproteolytic processing
prod_ft
chain 814..1030  (217) Cysteine protease (NS2 protein) (P23)
prod_ft
model3d 907..1030  (124) NS2-3 autoprotease catalytic domain domain (domain I) homology molecular model using template PDB:2hd0  
standard_name NS2
mat_peptide
 3431..5323
(1893)

NS3
function NS3 protein coding sequence (ns3 sequence)
locus_tag HCVORF1
product NS3 protein. Produced by autoproteolytic processing
prod_ft
chain 1031..1661  (631) NS3 protein (P70) [Includes: N-ter serine protease; C-ter NTPase and RNA helicase]
prod_ft
domain 1031..1210  (180) NS3 protease domain
prod_ft
domain 1217..1661  (445) NS3 helicase domain
prod_ft
model3d 1033..1661  (629) NS3 protein homology molecular model using template PDB:1cu1A  
standard_name NS3
mat_peptide
 5324..5485
(162)

NS4A
function NS4A protein coding sequence (ns4a sequence)
locus_tag HCVORF1
product NS4A protein. Produced by proteolytic processing by the HCV NS3 protease
prod_ft
chain 1662..1715  (54) NS4A protein (P8)
prod_ft
domain 1682..1693  (12) NS3 protease cofactor
prod_ft
model3d 1682..1693  (12) NS4A homology molecular model using template PDB:1cu1Z  
standard_name NS4A
mat_peptide
 5486..6268
(783)

NS4B
function NS4B protein coding sequence (ns4b sequence)
locus_tag HCVORF1
product NS4B protein. Produced by proteolytic processing by the HCV NS3 protease
prod_ft
chain 1716..1976  (261) NS4B protein (P27)
standard_name NS4B
mat_peptide
 6269..7666
(1398)

NS5A
function NS5A protein coding sequence (ns5a sequence)
locus_tag HCVORF1
product NS5A protein. Produced by proteolytic processing by the HCV NS3 protease. NS5A seems to have a transcriptional activatory role
prod_ft
chain 1977..2442  (466) Serine phosphoprotein (NS5A protein) (P58)
prod_ft
site 2213..2248  (36) Interferon sensitivity determining region (ISDR)
prod_ft
domain 2213..2274  (62) PKR binding domain
prod_ft
site 2326..2334  (9) Nuclear localisation signal (NLS)
prod_ft
site 2357..2383  (27) Variable region V3
prod_ft
model3d 2012..2174  (163) NS5A zinc-binding domain (domain I) homology molecular model using template PDB:1zh1  
standard_name NS5A
mat_peptide
 7667..9439
(1773)

NS5B
function NS5B protein coding sequence (ns5b sequence)
locus_tag HCVORF1
product NS5B protein. Produced by proteolytic processing by the HCV NS3 protease. NS5B is a RNA-dependent RNA polymerase that plays an essential role in virus replication
prod_ft
chain 2443..3033  (591) RNA-dependent RNA polymerase (NS5B protein) (P68)
prod_ft
model3d 2445..3008  (564) NS5B RNA-dependent RNA polymerase homology molecular model using template PDB: 1c2pB  
standard_name NS5B
3'UTR
 9443..>9589
(147)

3UTR
function 3'UTR (contains a polypyrimidine stretch and the conserved X region)
standard_name 3UTR
[General] [References] [Features] [Sequences] [Text Entry]    [LANL]
SEQUENCES
Sequence VIEW
Translation(s)
locus_tag HCVORF1 VIEW
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